Part:BBa_K494002
Arabinose-inducible mCherry Generator
In general we want to provide a new, scalable principle of gene regulation based on termination and antitermination which can be further developed, tested and optimizted by everybody. To enable that in the easiest way possible we decided to not just add the switches we designed but instead the material needed for evaluation. Therefore we focus on providing the parts needed for verification and testing of new individual switches.
With this BioBrick we provide a backbone which allows further cloning to test individual switches which can be easily designed using the principles described in the [http://2010.igem.org/Team:TU_Munich/Project TU Munich iGEM 2010 wiki].
BBaK494002 is intended as a positiv control for use with BBa_K494001.
This is a ready to use composite part consisting of the backbone BBa_K494001, a modified version of pSB1A10, the PBad Promotor BBa_I13453 and the reporter protein mCherry BBa_J06702 including RBS and double Terminator BBa_B0015. BBa_K494001 serves as a positive control: Without a terminator in between eGFP and mCherry and an additional promoter in front of mCherry, BBa_K494002 allows measuring mCherry fluorescence upon induction and comparison to the eGFP fluorescence. With BBa_K494001, measurements can be standartized to an internal positive control and it allows verification of induction, measurement consitions and expression kinetics.
Since BBa_K494001 can be used for evaluation of terminators and PoPS devices in general, BBa_K494002 can be used as a control in all those applications.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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